Journal: Cell Host & Microbe

Article Title: Complement C4 Prevents Viral Infection through Capsid Inactivation

doi: 10.1016/j.chom.2019.02.016

Figure Lengend Snippet: C1 and C4 Prevent Adenoviral Protein VI Exposure and Endosomal Escape (A) IP of Ad5 with 9C12 after Ad5 was incubated at the indicated temperatures for 30 min. WB: anti-adenovirus. (B) IP after Ad5 and 9C12 were complexed with C1 or C1/C4 and then incubated at 37°C or 49°C for 30 min. WB: anti-adenovirus. (C and D) HeLa TRIM21 KO cells were infected for 30 min in the presence of 9C12 or 9C12+ complement. Error bars depict the mean ± SEM of the indicated number of cells (n) acquired in three independent experiments. Scale bar, 5 μm. (C) Left: Ad5 staining is displayed in green; protein VI staining is depicted in red. Right: quantification of protein VI puncta per cell in the indicated conditions. (D) Left: Ad5 staining is displayed in green; Galectin-3 staining is depicted in red. Right: quantification of Galectin-3 puncta per cell in the indicated conditions. Original western blots are included in Figure S6 .

Article Snippet: Z-stacks with 1 μm thick optical slices were acquired and protein VI and Galectin 3 puncta were quantified using NIS Elements 4.30 (Nikon).

Techniques: Incubation, Infection, Staining, Western Blot

Journal: JHEP Reports

Article Title: Selective disruption of NRF2-KEAP1 interaction leads to NASH resolution and reduction of liver fibrosis in mice

doi: 10.1016/j.jhepr.2022.100651

Figure Lengend Snippet: Markers of liver fibrosis are reduced in response to S217879 treatment in DIO NASH mice. (A) Col1A1- and αSMA-stained liver sections at termination (n = 14 per group). (B) Quantification of Col1A1- and αSMA-positive areas by morphometry. Liver hydroxyproline levels quantified after extraction and centrifugation using a colorimetric assay. Data expressed as mean ± SEM. Significant differences were analyzed using Student’s t test. ∗∗∗ p < 0.001 and ∗∗ p < 0.01: S217879-treated vs. vehicle.

Article Snippet: Type 1 collagen (Southern Biotech, #1310-01), galectin-3 and alpha smooth muscle actin (αSMA, Abcam, Ab124964) immunohistochemistry were performed using standard procedures.

Techniques: Staining, Centrifugation, Colorimetric Assay

Journal: International Journal of Molecular Sciences

Article Title: Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells

doi: 10.3390/ijms21207555

Figure Lengend Snippet: Alpha-smooth muscle actin (α-SMA) and galectin-3 in LX-2 cells expressing chemerin isoforms. ( a – c ) LX-2 cells were transfected with an insertless plasmid (C) and plasmids to express huChem-155, 156 and 157. Cellular α-SMA was analyzed by immunoblot at 24, 48 and 72 h post-transfection. Coomassie stained membrane served as control; ( d – f ) Galectin-3 in the cells described in a–c; ( g – i ) Galectin-3 in the cell media of LX-2 cells described in a–c. N = 3; * p < 0.05, ** p < 0.01, *** p < 0.001, the number in ( h ) in brackets is the p -value.

Article Snippet: The antibodies used were obtained from the following sources: human chemerin, R&D Systems (Wiesbaden-Nordenstadt, Germany); GAPDH, New England Biolabs GmbH (Frankfurt am Main, Germany); galectin-3, BD Biosciences (Heidelberg, Germany); α-SMA and CMKLR1, Abcam (Cambridge, UK); GPR1, Bioss (Woburn, MA, USA) and Abnova (Taipei City, Taiwan).

Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot, Staining

Journal: International Journal of Molecular Sciences

Article Title: Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells

doi: 10.3390/ijms21207555

Figure Lengend Snippet: Secretome of HSCs and LX-2 cells. ( a ) IL-6 in the supernatant of HSCs of three different donors and LX-2 cells; ( b ) Pentraxin 3 (PTX3); ( c ) IL-8; ( d ) galectin-3; and ( e ) chemerin in the cell culture media of these cells.

Article Snippet: The antibodies used were obtained from the following sources: human chemerin, R&D Systems (Wiesbaden-Nordenstadt, Germany); GAPDH, New England Biolabs GmbH (Frankfurt am Main, Germany); galectin-3, BD Biosciences (Heidelberg, Germany); α-SMA and CMKLR1, Abcam (Cambridge, UK); GPR1, Bioss (Woburn, MA, USA) and Abnova (Taipei City, Taiwan).

Techniques: Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells

doi: 10.3390/ijms21207555

Figure Lengend Snippet: Effect of huChem-157 on IL-6, IL-8, pentraxin 3 (PTX3) and galectin-3 in primary human HSCs of three donors. ( a ) HSCs were incubated with increasing concentrations of recombinant huChem-157 for 24 h and IL-6; ( b ) IL-8; ( c ) PTX3 and ( d ) galectin-3 were measured by ELISA.

Article Snippet: The antibodies used were obtained from the following sources: human chemerin, R&D Systems (Wiesbaden-Nordenstadt, Germany); GAPDH, New England Biolabs GmbH (Frankfurt am Main, Germany); galectin-3, BD Biosciences (Heidelberg, Germany); α-SMA and CMKLR1, Abcam (Cambridge, UK); GPR1, Bioss (Woburn, MA, USA) and Abnova (Taipei City, Taiwan).

Techniques: Incubation, Recombinant, Enzyme-linked Immunosorbent Assay